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Back Wastewater monitoring for the presence of SARS-CoV-2 virus in the Slovak Republic – progress on 35th calendar week 2024 - RT-PCR and ddPCR analysis

Monitoring of wastewater in the Slovak Republic for the presence of SARS-CoV-2 virus was launched on 02.05. 2021. Since then, samples of waste water from evenly distributed waste water treatment plants (WTP) have been continuously examined. During the year, the monitoring intensity is optimalized according to the epidemiological situation.

The presence of virus in wastewater samples is determined by RT-PCR and digital dd-PCR. Samples are taken in cooperation with individual operators of wastewater treatment plants, regional public health authorities and investigated in the NRC at the Public Health Authority of Slovak Republic.

By the 35th calendar week 2024, a total of 5082 samples were examined by a qualitative and quantitative method, of which 4892 (96,26 %) samples were positive or with limit values.

On 35th calendar week 2024, 22 samples were examined, 22 samples were positive.  

Development of WW sample positivity rate in time

On the 35th calendar week, we noticed a small reduce in quantitative tests, which express the copy number of SARS-CoV-2 RNA.

Average RNA copy number of SASR-CoV-2 RNA in wastewater samples

Monitoring of the viral load in wastewater in individual regions points to a increase in Trnava, Žilina, Trenčín and Košice region.

Development of the incidence of COVID-19 disease and company number of SARS-CoV-2-RNA

SARS-CoV-2 in wastewater in regions

SARS-CoV-2 in wastewater in district